Enterobacteriaceae Identification

Laboratory testing algorithm for E. coli, Enterobacter aerogenes, Klebsiella pneumoniae, Serratia marcescens, Proteus vulgaris, and Proteus mirabilis

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Gram Stain
Gram Negative Rods

Oxidase Test
Negative

Enterobacteriaceae

MacConkey Agar
Positive Lactose - - Positive indole

Escherichia coli

MacConkey Agar - Positive Lactose
Indole Negative - - Urea Negative

Enterobacter aerogenes

MacConkey Agar - Positive Lactose
Indole Negative - - Urea Positive

Klebsiella pneumoniae

MacConkey Agar - Growth
H₂S Negative
Negative Lactose - - Citrate Positive

Serratia marcescens

MacConkey Agar - Negative Lactose
H₂S Positive - - Indole Positive

Proteus vulgaris

MacConkey Agar - Negative Lactose
H₂S Positive - - Indole Negative

Proteus mirabilis